Instead of removing the excess primers and dNTPS or breaking them down enzymatically, we have found that diluting with water is cheaper, easier and faster. Our PCR reaction (see Polymerase Chain Reaction protocol) does not call for a huge excess of primers or dNTPs. Our experiments have shown that the best way to prepare out PCR samples for sequencing is to dilute them with water. (See Polymerase Chain Reaction Protocol.) Terminator sequencing is used when first pass sequencing reveals an indel. In general, we use primer sequencing for all first pass sequencing attempts. The dilution of PCR products in preparation for sequencing.The preparation of pre-made sequencing plates.This protocol outlines the three steps involved in sequencing a PCR reaction: Big Dye Primer Sequencing Reaction Protocol Big Dye Primer Sequencing Reaction Protocol Overview
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